I'm trying to scale-up my post-PCR gels. I have access to a Biorad ICycler qPCR machine, but I also need to see if the PCR product is the right size, so I run the samples on gels. I currently use Invitrogen 10% acrylamide TBE gels, which give beautiful results but are expensive and limited to 15 or 30 samples at a time.
In general, I've been unhappy with old school agarose gels which give fuzzy bands. But money is also getting tighter.
Since I'm likely to need 100 wells at a time, and I like sharp bands, what agarose gel products do you recommend?
Agarose (which brand and what % for 50bp to 700bp), gel box, combs, other suggestions to speed things up and get great results?