RT-PCR NIBSC controls

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phani9999
phani9999's picture
RT-PCR NIBSC controls

Dear All,

We have recently procured NIBSC controls for HIV,HBV,HCV in Lyophilyzed form.We are thinking to use them for our routine analysis.

We are thinking to use them as high positive and low positive controls.Can any one suggest me what is the best method to extract DNA/RNA from Lyophilyzed plasma and best method to do dilution weather diluting the plasma or DNA diluiton

Regards,
Phani