DNA dilution

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jem
jem's picture
DNA dilution

 
I have an oligonucleotide stock solution of 1000nmol/mL. What volume of this stock solution must I get to obtain 5 nmols of oligonucleotide? Can you help me with this please? thanks!! :))

Chin Fen Teo
Chin Fen Teo's picture
Hi jem,

Hi jem,

Since you start with 1000nmol per 1 ml (or 1000 nmol per 1000 ul), the concentration = 1 nmol per ul. Hence, by taking 5 ul from your stock, it will contain 5 nmole of your oligo.