I'm wondering if anyone has knowledge about Tragacanth gum for cryostat use. I'm working with mice hearts, and read a paper in which the authors stated that hearts were extracted, mounted in tragacanth (5%), then flash frozen in cool isopentene. Does anyone know if the entire heart needs to be covered in tragacanth (after orienting it properly), then flash frozen or... is the tragacanth just used to hold the heart in place, then it is covered by OCT, then frozen?
I'm reading different protocols which contradict each other, (ie: cover / don't cover the sample in tragacanth). I wrote to the authors, who weren't very helpful..
Thanks for your time.