I am currently working with extremely small tissue samples (isolated muscles from mice pups) that are already hard to see. I find that after incubating in xylene, I cannot see the tissue and somehow end up losing some samples when changing solutions. Is there a stain to help me visualize my tissue to prevent this loss and to aid in orientation in the paraffin block? I would prefer that it not affect my immunofluorescence results.
Losing small tissues during processing after xylene step