Trouble shoot IFA technology (autoimmune medical diagnostic)

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Maestro
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Trouble shoot IFA technology (autoimmune medical diagnostic)

Appreciate some help. Does anyone know why a film over the slides that does not allow samples and conjugate to bind the substrate? In some wells the tissues bind perfectly in the first incubation, second incubation tissue totally impermeable and bind to nothe. The sections show at end of procedure a grey-orange color and no reaction. OTC is used on the slide.

Thanks