time interval for BrdU injection

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Shahin
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time interval for BrdU injection

Hi, to examine cell proliferation in tissues obtained from in vivo studies we normaly inject BrdU twice in the concentration of 50 mg/kg. Once 16 hour and once 2 hours before termination. In some cases we use the injection time 2 nad 7 hours. My question is that what is the importance of the gap time between 2 injections? Noting that we are working with cartilage tissue. Thanks in advance