I'm trying to find out if is there any difference between Sybr 14 and Sybr Green I for cell DNA/nuclei staining...? I need to evaluate sperm viability, but all the protocols I find use Sybr14 (available in Live/dead sperm viability kit, from Molecular Probes). Since I already have PI and Syber Green I in my lab, I was thinking if this combination would also suit the purpose... Most of the protocols I found for Sybr green I in cytometry were for microbial staining, not for sperm or mammalian cells. I think I'll try it anyway, while I wait for Molecular Probes' Kit (here in Brazil sometimes it takes long for some material to be delivered...).
Sybr14 x Sybr Green I