Phosphate Buffered Saline (PBS)

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Tony Rook
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Phosphate Buffered Saline (PBS)

preparation method for:

Phosphate Buffered Saline (PBS) 10X PBS (0.1M PBS, pH 7.2):

Na2HPO4 (anhydrous) --------------------- 10.9 g
NaH2PO4 (anhydrous) ---------------------- 3.2 g
NaCl ------------------------------------------- 90 g
Distilled water ----------------------------- 1000 ml

Mix to dissolve and adjust pH to 7.2 Store this solution at room temperature.

Dilute 1:10 with distilled water before use and adjust pH if necessary.

20X PBS (0.2M PBS, pH 7.2):

Na2HPO4 (anhydrous) ---------------------- 21.8 g
NaH2PO4 (anhydrous) ----------------------- 6.4 g
NaCl ------------------------------------------- 180 g
Distilled water ------------------------------ 1000 ml

Mix to dissolve and adjust pH to 7.2 Store this solution at room temperature.

Dilute 1:20 with distilled water before use and adjust pH if necessary.

10X PBS-Tween 20 (0.1M PBS, 0.5% Tween 20, pH 7.2):

Na2HPO4 (anhydrous) -------------------- 10.9 g
NaH2PO4 (anhydrous) --------------------- 3.2 g
NaCl ------------------------------------------- 90 g
Distilled water ----------------------------- 1000 ml

Mix to dissolve and adjust pH to 7.2 and then add 5 ml of Tween 20 Store this solution at room temperature.

Dilute 1:10 with distilled water before use and adjust pH if necessary.

20X PBS-Tween 20 (0.2M PBS, 1% Tween 20, pH 7.2):

Na2HPO4 (anhydrous) ---------------------- 21.8 g
NaH2PO4 (anhydrous) ----------------------- 6.4 g
NaCl ------------------------------------------ 180 g
Distilled water ------------------------------ 1000 ml

Mix to dissolve and adjust pH to 7.2 and then add 5 ml of Tween 20 Store this solution at room temperature.

Dilute 1:20 with distilled water before use and adjust pH if necessary.

Note: PBS buffer is used for routine immunohistochemiscal staining. PBS is often used for diluting secondary antibodies or streptavidin-HRP conjugate. PBS-Tween 20 is often used for washing steps. Link Reference: http://www.ihcworld.com/_protocols/washing_buffers/pbs.htm

riturajz
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if i want make the sorensen's

if i want make the sorensen's buffer for pH: 6.8 ( 3 to 4.8 M ) what qty. i have to add of di-sodium hydrogen phosphate and pottasium dihydrogen phosphate

Bandara
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Thank you for  registering me

Thank you for  registering me . bandara

Arvind Singh Pundir
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welcome Bandara to SSI,

welcome Bandara to SSI,

 post your queries  on this board

bimalnpj@yahoo.com
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 sir i need the chemical

 sir i need the chemical composition hydrous PBS ph is 7.4

Arvind Singh Pundir
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 Hi bimal,

 Hi bimal,
are you asking about PBS with hydrous phosphate then composition for 10X PBS is as 
NaCl  -   80gm
Na2HPO4.2H2O- 18gm
KCl-  2 gm
KH2PO-  2.4gm

pH 7.4-7.5