Please find the following media preparation method:
Gill's Hematoxylin Solution
Chemicals Single Double Triple Function
Ethylene glycol 250 ml 250 ml 250 ml solvent
Distilled water 750 ml 750 ml 750 ml solvent
Hematoxylin 2 g 4 g 6 g dye
Sodium iodate 0.2 g 0.4 g 0.6 g oxidizing agent
Aluminum sulphate 20 g 40 g 80 g mordant
Glacial acetic acid 20 ml 20 ml 20 ml pH control
Dissolve ethylene glycol in distilled water.
Add hematoxylin, then sodium iodate.
Add aluminum sulphate and acetic acid
Stir for 1 hour at room temperature.
Filter if necessary.
Staining Characteristic - Progressive
Single strength for cytology.
Triple strength for immunohistochemistry counterstain (dilute 1:5) for 30 seconds and H&E (dilute 1:5) for 5 minutes.
This recipe gives clear and sharp nucleus staining with little background. Good for both immunohistochemistry counterstain and H&E. This solution is also alcohol and mercury free so it is good for IHC counterstain when AEC substrate is used. When triple strength is used, dilute 1:5 with distilled water before use.