Why not in BL21

1 post / 0 new
rajendra's picture
Why not in BL21

 Need help ....

I cloned a gene in pET23a vector and when I transformed into DH5alpha I got successful clone.
but when I'm transforming the same clone into BL21(DE3), im not getting any clone.

Its very surprising to me...
bcz I checked BL21 for their transformation efficiency, they are fine.

How a clone able to transform iN DH5alpha, fails in BL21.

Im not even inducing their expression, juz transformation... 

Plz help me in understanding whats happening here.