What Bacteria for Transforming?

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Amtekoth
Amtekoth's picture
What Bacteria for Transforming?

I've got a >10kb low-copy # plasmid that I need to transform and grow up. What commercially available competent bugs would you recommend?
Not too expensive!

I'm mainly worried that some bacteria I've used in the past can't handle larger-sized plasmids.

Thanks,

Ed

Jason King
Jason King's picture
There are quite a few that

There are quite a few that should work but the ones I've used for adenovirus plasmids are Stratagene's XL10-Gold. Buy once then make them yourself (I've got a good protocol for making chemically competent cells - works great with XL-10s). These cells are also recommended for plasmids with repeat sequences - terminal repeats in viral vectors etc.

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