low protein expression

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chaowalit
chaowalit's picture
low protein expression

Dear all,
This ' s a big problem for me, I express the HuScFv protein using pcantab5e transfromed into E. coli TG1. In the first time, the protein was express high yeild but next time this protein low express. What should i do? please tell me. I need some help. Thank you

protoldo
protoldo's picture
Hola,first I never have used

Hola,first I never have used this plasmid and strain but using other plasmids and E coli host, for me this phenomenom occurs when you have used an old colony for preinoculum, try to seed from glycerolizated -80 strain or if you have done it transform newly. Good Luck

Shatarupa
Shatarupa's picture
Hola, I agree to the post

Hola, I agree to the post made earlier to mine. I have never worked with the plasmid you mentioned, but I have found in these cases, you can take from the fresh stock at -80 C ,do transformation/ isolate Plasmid DNA and then try for protein expression. That is start afresh from a fresh new stock at -80 C.

chaowalit
chaowalit's picture
Thanks a lot. first, I'll

Thanks a lot. first, I'll take E. coli from glycerol stock culture.

StephenJones1
StephenJones1's picture
Hi friend,

Hi friend,
Diet high in proteins has been related to weight loss and weight maintenance in various recent studies. Protein keeps the body satisfied for a longer time, the feeling of satiety. There is convincing evidence that a higher protein intake increases thermogenesis (the process of burning fat to produce body heat) and satiety as compared to diets of lower protein content.

Thanks
DNA Methyl transferase