PXR reporter gene assay

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boguslav
boguslav's picture
PXR reporter gene assay

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I am an analytical scientist in DMPK group. We are currently trying to establish a reporter gene assay for CYP induction assessment. I have a very limited biology experience, transfection part is very tricky to me.
Can we buy PXR and AhR transfected cryo cells or do we have to perform our own transfection?

R Bishop
R Bishop's picture
Mammalian cell transfection

Mammalian cell transfection is becoming fairly routine, if you pick a standard cell line like Hela or HepG2 you should have no problem transfecting a commericially available construct PXR activity.  Many companies make transfection reagents with easy detailed protocols for standard cell lines.

Bishop