Lactobacillus promoter activity

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csteiche's picture
Lactobacillus promoter activity

I have recently been tasked with characterizing the activity of a lactobacillus promoter.  I don't have any experience with this specifically.  I was thinking about just fusing my promoter of interest to the Beta galactosidase gene in a low copy plasmid and analyze expression under various conditions.  However, LAB are difficult to lyse.  I am looking for any suggestions on other options to assess bacterial promoter activity under various conditions.  Thank you