E. coli Strain for pHANNIBAL and pART27

6 posts / 0 new
Last post
nucleo77
nucleo77's picture
E. coli Strain for pHANNIBAL and pART27

Dear All,

What is the E. coli strain that could be used to host the pHANNIBAL vector developed by CSIRO of Australia? Thanks

behrooz
behrooz's picture
E.coli k12

E.coli k12

behrooz
behrooz's picture
Hi, Nucleo77

Hi, Nucleo77
the strain that you applied was derived from k12.do you use from correct method for transformation? it is possible because of bad transformation or medium elements, they will began to destroyed. also you must add appropriate antibiotic(s) at medium, did you add any? I think ampicilin is the appropriate antibiotic that you add with 1mg/mL concentration.
with best greetings behrooz

rosemary
rosemary's picture
hi, sir, where did you get

hi, sir, where did you get this two vector? I need use this too. Could you please email me at eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%72%6f%73%65%5f%75%73%35%32%30%40%68%6f%74%6d%61%69%6c%2e%63%6f%6d%2e%54%68%61%6e%6b%73%22%3e%72%6f%73%65%5f%75%73%35%32%30%40%68%6f%74%6d%61%69%6c%2e%63%6f%6d%2e%54%68%61%6e%6b%73%3c%2f%61%3e%27%29%3b'))!

What is the E. coli strain that could be used to host the pHANNIBAL vector developed by CSIRO of Australia? Thanks

ayush.chowdhry
ayush.chowdhry's picture
Sir can E.coli DH5 alpha be

Sir can E.coli DH5 alpha be used for pHANNIBAL. If not what other strains can be used?

The FFM
The FFM's picture
 pART27 was described in this

 pART27 was described in this paper

 The map can be found in this reference

Gleave AP: A versatile binary vector system with a T-DNA organizational structure conducive to efficient integration of cloned DNA into the plant genome.
Plant Mol Biol 1992, 20:1203-1207. PubMed Abstract | Publisher Full Text 

http://www.springerlink.com/content/w350542n1224856j/fulltext.pdf

It's old buy you might try writing to him to see if you can obtain a sample