Plant leaf agroinfiltration

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ralf's picture
Plant leaf agroinfiltration

I'm using the agroinfitration method to transiently express tagged proteins in N.benthamiana leaves. The Agrobacterium causes a microscopic damage to the cells by creating "bubbles" between the cells of injected leaf (like separating the walls of adjasent cells).
Did anybody encounter this problem, and any suggestions to avoide this?