pkc activity assay

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Pinal Pandya
Pinal Pandya's picture
pkc activity assay

hi all,
i know that when protein kinase C is activated, translocates to the membrane where it is bound and phosphorylate surrounding molecules. But i wanted to do an activity assay and to get maximum level of activity i was thinking of doing an extraction step which will dislocate the enzyme and release it in the cytosol, which could be measured. SO my question is do u think that by extaction step, the pkc will be rendered inactive and i will be measuring non- active enzyme in the end??? Does that make sense? I am very new to this area so help me out please!!!

thanks

saswati1
saswati1's picture
HI, I was wondering whether

HI, I was wondering whether you have tried determination of PKC activity in membrane and cytosolic fraction by using an Amersahm kit. Here is the paper to follow

Original Article

Neuropsychopharmacology (2002) 26 216-228.10.1038/S0893-133X(01)00327-X

Pinal Pandya
Pinal Pandya's picture
hi saswati1

hi saswati1
i really appreciate your help and i did get the paper you sent me. will write my feedback once i figure this out. Thank you very much

saswati1 wrote:

HI, I was wondering whether you have tried determination of PKC activity in membrane and cytosolic fraction by using an Amersahm kit. Here is the paper to follow

Original Article

Neuropsychopharmacology (2002) 26 216-228.10.1038/S0893-133X(01)00327-X