I am working with thyrocytes and are trying to get a nice dose/response with the U0126 MEK inhibitor.
I do not get that terrific results, especially they are not that consistent between different experiments,
So, when serching the net for different thoughts I realize that many authors (and also the manufactorer...obviously) often prestimulate for 30 min - 4h before stimulation.
What are your opinion? (or even better experience)?
could such a thing actually affect the results?
I do my stimulations for 48h...