phosphorylated ERK

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Sandy
Sandy's picture
phosphorylated ERK

I am setting up experiments that involve phosphorylated and total ERK calculations.
I heard about colorimetric and chemiluminescent immunometric products that are available.
Can any one recommends a company with a product they have used?

roudi
roudi's picture
Sandy wrote:I am setting up

Sandy wrote:

I am setting up experiments that involve phosphorylated and total ERK calculations.
I heard about colorimetric and chemiluminescent immunometric products that are available.
Can any one recommends a company with a product they have used?

Dear Sandy,

I can think of two companies: 1- A company called : Active Motif, Inc. at www.activemotif.com with a product called FACE or Fast Activated Cell-based ELISA to analyse protein phosphorylation.
2- A technology developed at DiscoveRx commercialized under the name HitHunter HTS assays for GPCRs and Kinases.
using chemiluminescent signals.

I hope it helped.

xz35
xz35's picture
The ERK ELISA kit from Active

The ERK ELISA kit from Active Motif, Inc is fine. Give a try.

RH
RH's picture
active motif. It is good.

active motif. It is good.

nin1318
nin1318's picture
if you are interested in

if you are interested in doing quantitative immunoblotting there are some good antibodies to regular and phospho-erk...i cannot remember now exactly who they were from since it was a while ago that i used them, but i am pretty sure that one of them was from santa cruz.

nin1318
nin1318's picture
i know it's been a while

i know it's been a while since you posted this inquiry, but i just noticed today and ad on the back of the scientist for their cell signaling assays. if you buy one and use it and tell them the results they send another one free (though the issue is from 2/28 so the offer might not still be on). anyway, one of the listed kits is ELISA based total and phosphospecific erk.

sigma-aldrich.com/assaykits_ny3

Stix
Stix's picture
Sigma does a great phospho

Sigma does a great phospho-ERK1/2 antibody (WB 1:1000) always worked for me. Use with ECL+ reagents (Amersham) for quantitative western blotting if you have a STORM imager for scanning blots directly by chemifluorescent detection (image is saved directly and can be quantified using associated IMAGEQUANT software). Santa cruz ERK2 antibody is good for total ERK expression (WB 1:2000)

mchinmoyee
mchinmoyee's picture
I regularly use immunoblot

I regularly use immunoblot and quantitatively take IDV from the chemilimunisence developed hyperfilms or from Gel-doc software itself.
If you need any specific information check methods here:

Sharma SK, Bagnall MW, Sutton MA, Carew TJ.
Inhibition of calcineurin facilitates the induction of memory for sensitization in Aplysia: requirement of mitogen-activated protein kinase.Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4861-6. Epub 2003 Apr 2.