Hi, I'm western blotting on phospho-mapk and mapk.
Some antibodies show me the figure of one band or sometimes double while reading a paper.
what was that?
if double, the upper and the below are going together at the same expression level in western blotting?
I mean, I'm stripping the membrane after detecting the phospho-form first to re-detect the non-phospho form of mapk.
Otherwiese, is it possible to see if the upper only decreases while the below has no change in density? or vice versa?