Does K252a blocks MAPK activation?

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mchinmoyee
mchinmoyee's picture
Does K252a blocks MAPK activation?

Can anybody tell me about there observation using K252a a kinases inhibitor, works as RTK inhibitor at 200nM , and not blocking Mapk activation.
Thanks

Lushi
Lushi's picture
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Celia Cruz
Celia Cruz's picture
wrote:

wrote:

You have to be careful. ERK can be activated not only by Trk receptors (blocked by K252a) but also by calcium entry. So, we have to be sure if you don't have calcium entry in your cells that would activate ERK.

mchinmoyee
mchinmoyee's picture
So, it means if a system

So, it means if a system which allows Ca++ entry will not be compatible for using K252a as a blocker of Trk Receptor, what else can one use to check the receptor Trk B involment?

and does anybody have observed that MAPK is blocked using K252a and at which conc.?

Thank you