Monitoring Kinase activity

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omid
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Monitoring Kinase activity

Not every Kinase will work in an in vitro assay and a peptide that is phosphorylated by a kinase in vitro does not necessarily reflect that kinase's physiological target. However, kinase kits provide efficient way to monitor the activity of a kinase and screen compounds that will affect this function.

I know about a platform offered by Invitrogen called Z-LYTE kinase assay. the detection method relies on the proximity of two fluorescent dyes. Does anyone know about this kit and others available in the market?

DD
DD's picture
omid wrote:Not every Kinase

omid wrote:

Not every Kinase will work in an in vitro assay and a peptide that is phosphorylated by a kinase in vitro does not necessarily reflect that kinase's physiological target. However, kinase kits provide efficient way to monitor the activity of a kinase and screen compounds that will affect this function.

I know about a platform offered by Invitrogen called Z-LYTE kinase assay. the detection method relies on the proximity of two fluorescent dyes. Does anyone know about this kit and others available in the market?

I haven't heard about the Z-LYTE kinase kit but I know the following companies offer very interesting products for Kinase targets:
Novartis(www.novartis.com)has a kinase inhibitor called: Gleevec.
Axxima Pharmaceuticals (www.axxima.com) and DiscoveRx ( www.discoverx.com) have similar products.