Inhibitor - activation?

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sharmakaushikp
sharmakaushikp's picture
Inhibitor - activation?

In case of inhibitor experiments (Protein kinase inhibitors for the dissection of signal transduction pathways), how do we explain the fact when an inhibitor itself produces some changes (either up-regulation or down-regulation) different from the basal level/control.
for example, if a experiment consists of 4 groups- control C, treatment T, inhibitor I, inhibitor + treatment I+T, and if the inhibitor itself in this case produces changes different from the control, how do we explain this observation?

Fraser Moss
Fraser Moss's picture
This means that in the basal

This means that in the basal state, the activity of the target of the inhibitor is not 0 and that the inhibitor is changing the basal activity when compared to the no drug control condition

saswati1
saswati1's picture
As long as you use proper

As long as you use proper positive and negative controls, even though the inhibitor itself makes changes, still you can justify your results

sharmakaushikp
sharmakaushikp's picture
So in the case when the

So in the case when the inhibitor itself is affecting the basal activity, even at different (lower) concentrations, then how do we analyze the effect of the inhibitor as in whether the inhibitor is affecting the activation by the particular treatment or not?
When I am looking at fold activations, what would be the appropriate way to find meaningful information from the experiment during analysis of the data?