Dye Transfer: Any cell permeable dye?

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thebimshi
thebimshi's picture
Dye Transfer: Any cell permeable dye?

Have anyone tried dye transfer (esp. in cardiomyoctyes) using cell permeable dye? (so that I don't have to puncture the cells)
I don't want to use cell impermeable dye like Lucifer Yellow or Ethidium Bromide?
Thank you very much =)

saswati1
saswati1's picture
What is the purpose of teh

What is the purpose of teh dye? Do you want to stain specific organelle?

Abhishek Singh
Abhishek Singh's picture
yes let me know such dye i

yes let me know such dye i want to do live cell imaging

Arvind Singh Pundir
Arvind Singh Pundir's picture
Hi Abhishek,

Hi Abhishek,
if you want to do live cell imaging with dyee as probes you can go through the following paper which describes series of fluorescent compounds suitable for live cell imaging.
 "Cyanine dye conjugates as probes for live cell imaging" www.sciencedirect.com/science
 also refer the post on this site(SSI) and original refernce link, in which they have mentioned about "fluorogen activating proteins" (FAPs) The FAPs can be used to monitor biological activities of individual proteins and other biomolecules within living cells in real time www.cmu.edu/news/archive/2008/February/feb6_FAPs.shtml
www.scientistsolutions.com/t6173-fluorescent+proteins+developed+for+live+cell+imaging,+biosensor+design.html
 
 
 
 
 

heehawmcduff
heehawmcduff's picture
The celltracker dyes are
Abhishek Singh
Abhishek Singh's picture
there are various dye which

there are various dye which depend on esterase some dye like DiSC2, Fura 2AM for calcium and DHFDA are dye that are permeable but first define the urpose

Abhishek Singh
Abhishek Singh's picture
t hank you  that is worth for

t hank you  that is worth for me

heehawmcduff
heehawmcduff's picture
As well as Celltracker, the

As well as Celltracker, the Celltrace dyes are apparently very good for long term tracking of daughter populations.
http://products.invitrogen.com/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&productID=C34553