Tranfection of EL4 cells (EL4 (Mouse lymphoma cell line), please help

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ziseputi
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Tranfection of EL4 cells (EL4 (Mouse lymphoma cell line), please help

Tranfection of EL4 cells (EL4 (Mouse lymphoma cell line), please help

The gene was cloned into plasmid.

Could you please give me a protocol in details? Thanks a lot.
 
I heard It is not easy to trafect EL4 cells, These cells are floating,  not adhere to the flasks

I have succeeded in transfecting other cells, but do not have any experience in transfectin EL4, Could you please tell me your protocols if you happen to transfect this type of cells?