Hi all,
I am trying to intrduce my gene of interset by retroviral infection into MEFs. for this first I do transfection in platE cells and then collect the viral supernatant to infect MEFs. then i start the selection of infected MEFs. but the problem is, all my cells (MEFs) die during selection!!!!! I have tried everything till now, but nothing is working...
any suggestions will really welcome...
thanks in advance!