retroviral infection using platE cells

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geets
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retroviral infection using platE cells

 Hi all,

I am trying to intrduce my gene of interset by retroviral infection into MEFs. for this first I do transfection in platE cells and then collect the viral supernatant to infect MEFs. then i start the selection of infected MEFs. but the problem is, all my cells (MEFs) die during selection!!!!! I have tried everything till now, but nothing is working...
any suggestions will really welcome...

thanks in advance!