oNPG assay

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IRB's picture
oNPG assay

We are working on fibroblast senescence and we tried the oNPG assay to detect the AS-beta-galactosidase activity in human fibrblasts. We induce senescence with H202, but we are not able to detect anything significative. Probably we don't stimulate enough with H2O2 or we use few cells....
I would like to know if anyone has a protocol for this assay...
Thank you very much for your attention!!