I start working on HEK293 cells recently. I transiently transfect HEK cells with plasmids which encode an ion channel. I found many cells detach easily after 6h incubation with lipofectamine 2000. My cells are 60-70% confluence before transfection. I use 1.5 ug plasmids in 200 ul MEM, and 5 ul lipofectamine 2000 in 200ul MEM. MEM has no antibiotics or serum, I record whole cell current one day after transfection. My data are not good at all.
So, I am wondering whether cell lose after transfection affect data intergrity. Is there any good way to manage HEK cells, letting them adhere tightly to the dishes?
I would appreciate greatly for your valuable suggestions.