comet problem

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htbk's picture
comet problem

I have a problem that cells on microscope image aggregate side of slide and no cell image on the middle of slide.   

normal melting point agarose coating slides dont like homogen surface. in addition, backround of the slide look dirty and fuzzy in the course of cell imaging on the microscope.
after elektrophoresis, positive control dna tail not line, scattered around of core.

What can i do for this proplems. could you please give me information about this proplem,