Cell Lysis to obtain GFP

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biomongoose17
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Cell Lysis to obtain GFP

Hi

I need to lyse mammalian cells (Hela) to release GFP so that I can measure fluorescence on a microplate reader.  What is the best method to lyse the cells?  The paper I have uses freeze/thaw but does not mention buffer, any suggestions? Also, do I need protease inhibitors if I am just going to lysis the cells, read the fluorescence, then toss the plate?

I have never worked with proteins before so any help would be greatly appreciated.

Thanks!!