I want to start a new experiment to confirm results I obtained using a bacterial 2 hybrid system, which identified putative interactants of my gene of interest. Seemingly confirmation is done by immuno-precipitating your gene of interest with its putative interactant after stably expressing both in a eukaryotic cell line.
I have never done cell culture before. Can anyone help suggesting which cell lines would be best for this procedure? For instance: suspension or monolayer, which system is best to transfect, are certain cell lines better at expressing protein etc?