I transfected 293T with calcium phosphate kit from clontech. as soon as i add the transfection mix the medium turned cloudy and pink and a lot of black stuff floating around. the cells were almost fried the next day. this time the 293Ts were in McCoy's 5A medium. The one previous time when I did this, the cells were in DMEM when this problem did not occur.
are there anythoughts on this?