Can anyone help me with in what does Benfotiamine dissovles except pyridine:water( that Sigma recommends)
I surprised that it did not dissolve in DMSO, given that it is essentially a lipophilic thiamine.
I did find a report that crystallized it out of an ethanol solution - seems like Sigma also provides it in an Ethanol solution (or atleast it used to)
(Acta Cryst. (1993). C49, 294-298 [ doi:10.1107/S0108270192006905 ]
Structure of benfotiamine hemihydrate).
# Babaei-Jadidi R, Karachalias N, Ahmed N, Battah S, and Thornalley PJ. Prevention of incipient diabetic nephropathy by high-dose thiamine and benfotiamine. Diabetes 52: 2110–2120, 2003.[Abstract/Free Full Text]
# Babaei-Jadidi R, Karachalias N, Kupich C, Ahmed N, and Thornalley PJ. High-dose thiamine therapy counters dyslipidaemia in streptozotocin-induced diabetic rats. Diabetologia 47: 2235–2246, 2004.[CrossRef][ISI][Medline]
# Hammes HP, Du X, Edelstein D, Taguchi T, Matsumura T, Ju Q, Lin J, Bierhaus A, Nawroth P, Hannak D, Neumaier M, Bergfeld R, Giardino I, and Brownlee M. Benfotiamine blocks three major pathways of hyperglycemic damage and prevents experimental diabetic retinopathy. Nat Med 9: 294–299, 2003.[CrossRef][ISI][Medline]
I tried to get in touch with the scientists from the papers you suggested but in vain :(
I am totally clueless about Benfotiamine now !
Check out the first ref - they describe the EtOH/H2O solution, and contact Sigma to see if they can provide you with what that group used.
Sure will do !