Anybody having experience in HL-1 transfection?

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Shaymaa Ahmed
Shaymaa  Ahmed's picture
Anybody having experience in HL-1 transfection?

I have transfected my HL-1 cells using Fugen but the trasfection rate is low. I also wanted to ask whether fixing the cells with 2% paraformaldehyde lower the transfection rate?

Your suggestions are mostly valuable. Thanks in advance.