flow cytometry

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hanen ouadani
hanen ouadani's picture
flow cytometry

hello
when suspending cells in PBS for FACS analysis, why we add BSA or FBS to the PBS ?
thanks for help

marcus muench
marcus muench's picture
 Generally, cells are kept in

 Generally, cells are kept in low concentrations of FBS, BSA etc to help maintain viability.  Cells normally do not reside in a saline solution, but rather are bathed in protein and other compounds found in extracellular matrix, plasma or through cell-cell contact.
 
During the staining and washing procedures FBS or BSA may also help to act a blocking reagents to prevent non-specific binding of antibody.  
 
Too high a concentration of protein can cause some trouble or build-up in flow cytometers.  Mostly, I try to avoid more than a couple % FBS when cell sorting.  Addition of protein to the collection fluid also helps maintain viability.

hanen ouadani
hanen ouadani's picture
thank you :)

thank you :)

Nazunderwent
Nazunderwent's picture
i think this is for prevent

i think this is for prevent unspacific binding of antibodies in the solution and for the cells the proteins from outside orgin might have some enhancing signaling in order to prevent cell shock ..