FACS

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namitakanwar
namitakanwar's picture
FACS

Hi! I am trying to study immunological synapses (IS) formed between NK and target cells. I was wondering if it is possible to use FACS to quantify the number of NK-target conjugates formed between the cells under different conditions ? I am thinking of using surface markers to distinguish the homotypic and heterotypic conjugates. It seems very exciting but I am a bit skeptical because 1. the ISs are very fragile, so would they stand the sample preparation procedure as well as the FAC counter suction pressure etc? 2. People generally apply doublet removal in FACS an use only single cells for statistics, i was wondering if it possible to actualy pick doublets and study them and discard the singlets?