Cell loss during annexin staining/flow cytometry

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Sophie Irwin
Sophie  Irwin's picture
Cell loss during annexin staining/flow cytometry

 Hi,

I'm currently doing flow cytometry on neutrophil apoptosis. I'm seemingly having an issue with the number of events going through the flow, from 150 - ~3000 when I intially load 300,000 neutrophils at the start (I'm aiming for 10,000 events). I can't see why I have this issue as other users in the lab haven't had this problem

Any thoughts?

Sophie

Chin Fen Teo
Chin Fen Teo's picture
Hi Sophie,

Hi Sophie,

what is the source of neutrophil you use in your experiment? Did you observe any clumps while handling them?