Hi to All
I am working on MCF7 cell lines. I have recently started culturing them but unfortunately there are some black dots whuch seems like cell debris but i am not sure. Moreover, my cells are not seems to be happy. they are lacking their prominant cell wall i suppose. And i can see various dotted strtcture within the cell itself. there are no bacterial contamination i am sure about that. can anybody please suggest me any rectification or procedure so i can maintain my cells in healthy way. It is really very urgent. Kindly help.