Oil Red O Staining Problem

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SpringSun778's picture
Oil Red O Staining Problem

Hi All,
I just stained 3T3-L1 cells with Oil Red O. During the washing steps after staining, the undifferentiated cells washed right off the plate! The differentiated cells that had stained TGs stuck to the plate fine. I have never had this problem. Any suggestions?
I used 10% formalin to fix the cells
Thank you