INFLUENZA VIRUS CULTIVATION IN CELL LINE

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pavulraj
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INFLUENZA VIRUS CULTIVATION IN CELL LINE

 Hai, iam trying to cultivate equine influenza virus H3N8 in MDCK cell lines, but cells are detaching after 3rd day placing in maintenance media without adding serum, repaeted passages revealed same detachment problem. cells in growth medium with 10 percent serum - growing well without any problem, once placed in maintenance media and incubating fro 3 -4 days cells are detaching. what to do? please hepl me