Cell counting and MTT assay

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PriyaSar
PriyaSar's picture
Cell counting and MTT assay

 Hi  all..

I was doing MTT assay IN HUMAN LIVER cells. but it is not working well, the results are not appropriate in control and in treated cells.

Can anyone say me how to troubleshoot..or give the procedure and calculation for cell counting for hemocytometer..

waiting fr reply

thanks