Trouble with Soft Agar Assay

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PeeBee
PeeBee's picture
Trouble with Soft Agar Assay

Hello -

I have been having trouble doing the Soft Agar Assay, testing anchorage independent growth.
I have not been able to get any cells to grow at all.
Is it possible that I am killing the cells when I make the cell suspension?
I am plating 500 cells per well in a 6-well plate. 
Has anybody had success with this and knows how to troubleshoot?
Please help.
Thanks!