I have some question about angiogenesis method. I have a problem in experim

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swdbell
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I have some question about angiogenesis method. I have a problem in experim

I have some question about angiogenesis method. I have a problem in experiment.
I do Spheroid assay for 3D spheroid by Hanging drop technique. Using matrix by collagen from rat tails so my matrix is not clear   I think it participate of collagen,   because it has a white fibrous whan see in well and debris in microscope.
From this problem impact for spheroid is not good,not circle,       cell of this expose form there in next time so don't see abnormal shape in first day.         I think this problem happens form collagen hasn't well of polymerization.
How can do it ?       I do collagen on ice 3-5 minitue.

Thanks you

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