I am going to do some studies about angiogenesis assays in vitro for EPCs.After reading some papers ,I found many authors used the matrigel finished this assay. Because I have never made this assay before and there is no exac procedure for this assay in papers. So I want to know how to coat the 96-well cuture flask with matrigel. whether the cells need stained before seeding on the matrigel. How to get the effect like the picture showing.Thanks a lot for any suggestion.