So I am starting this new project on 3D culture, and practically have no knowledge about 3D culture system. To begin with, I'm (we are) planning to culture colorectal cell lines (HTC116) in 3D, and then move to primary culture of colorectal cells in 3D. I need a simple and cost-effective system to present some positive data to our director and get a nod for the direction I am thinking to take for the project. I wanted to know if it is possible to grow these cells into spheroids in agar coated plates or matrigel? If not, please suggest some alternatives. Are there any other method that could be easily established in the lab without having to spend heaps just in the beginning?
I read about breast cancer cell lines that can be easily grown in 3D and they start expressing tumor-like charateristics. Do only particular cells have this ability to form spheriod or any adherent cell line will form spheroids under suitable conditions? A
Lastly, is it possible to do MTT on spheriods grown in a 96-well plate to determine IC50 values?
Thanks a lot