3D cell culture

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samm
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3D cell culture

What are the 3D cell culture matrices you have tried out? Which one is your favorite? What do you use them for (invasion, cyst formation etc)?
Is it the layered collagen or matrigel or the new proprietary/synthetic matrices that are now available.

jabba
jabba's picture
Our lab has been using

Our lab has been using Extracel from Glycosan BioSystems. We've been growing hESCs and differentiating them into neurons. It's easy to use and works well. The only downside for us is that it is a new product, so there aren't as many protocols as there are for Matrigel or collagen.

JoshPRoberts
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I'm writing an article for

I'm writing an article for The Scientist magazine's Lab Tools section about 3D cell culture. I'm looking for users like samm and jabba -- and anyone else -- who may have challenges and/or solutions to 3D cell culture that they'd be willing to share. If that's you, please send me a private message with your contact information.

Thanks!
Josh

samm
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Hi Josh! sorry for the delay

Hi Josh! sorry for the delay -but let me know if you have any particular questions about the technique, or just want to get some impressions on setting up 3D cultures (painful - but when it works, the reward is in the pretty pictures: on the plus side, its way easier than organ cultures!).
-sam

p.s. There have been several post exchanges on the forum on this subject. Those might also help give you some idea about what researchers think of it, and the problems face (the biggest of those, in my opinion, is variation in the matrix, unless you are lucky enough to have cells that grow in a defined matrix like Invitrogen's new alginate based ones)

bob builder
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Has anyone tried 3D Insert

Has anyone tried 3D Insert from 3D Biotek?

Deirdre
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I am doing some market

I am doing some market research for a client that is developing a novel 3D cell culture matrix. I am curious if there is a need for more "customized" matrixes that more closely mimmic the ideal in vivo environment for a specific cell. My understanding is that the existing 3D matrices (e.g., Matrigel) provide a more "generic" 3D environment. Are there specific types of cell where a customized 3D environment would be most beneficial? Or cells where generic 3D matrices work fine?

Also, would a tool that could rapidly determine the ideal material and geometry for a specific cell matrix be of value? If so, for what applications?

Thanks for your insights

samm
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There are applications where

There are applications where you "customize" the matrix - which is mostly a trial and error process, often involving different ratios of components (e.g. collagen+Matrigel (which itself contains collagen!)) that show some degreeof batch-to-batch variability. Defined matrices that are currently available usually do not work with all cell types - and Murphy's law usually works in this case...

Deirdre
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Samm,

Samm,

From your perspective, why cell types are the current generic 3D cell culture least effective?

Thanks
Deirdre

samm
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Deirdre wrote:Samm,

Deirdre wrote:

Samm,

From your perspective, why cell types are the current generic 3D cell culture least effective?

Thanks
Deirdre

Hi Deirdre! I'm not sure if i understand your question properly, but to the best of my knowledge, there is no ONE matrix/protocol that I know of, that is suitable for all 3d/primary culture requirements.
Cells/tissues have very different microenvironments - which translates to different requirements for matrices, growth factors, media and culture conditions. Finally, different kinds of assays/cultures with the same tissue/cell type (a common eg would be cell growth and expansion v/s differentiation) can have different requirements. In addition, cells like L Ep TypII can actually digest and regenerate MatriGel/Collagen type matrices to "create" something on their terms!

jhardin
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Samm I an a recruiter in the

Samm I an a recruiter in the life science space wanted to see if we can connect and talk about 3d cell cultures and who you know and respect in the industry, my number is 703-729-5250

DasCopy
DasCopy's picture
samm wrote: What are the 3D

samm wrote:

What are the 3D cell culture matrices you have tried out? Which one is your favorite? What do you use them for (invasion, cyst formation etc)?
Is it the layered collagen or matrigel or the new proprietary/synthetic matrices that are now available.
 

 
I have 5 years experience using OptiMaix collagen scaffolds offering a uniquely open porous structure which allows very nice cell seeding efficiancy.
 
Greetings,
Sven
 

heehawmcduff
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http://www.millipore.com

http://www.millipore.com/cellbiology/cb3/ecmcoating
A colleague of mine uses matrigel coated millicells.

minylim
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I've tested collagen type I,

I've tested collagen type I, fibronection and matrigel. I wanted to try soft agar too, but my boss stopped me before I 'waste' time. I'd definitely vote for matrigel. I've tested a couple of cell lines under different conditions, many more cells grow happily on matrigel than other matrix, at least they don't die. I know there's some soft biological matrix from algae (kind of...not quite sure) but I doubt it. I'm in trouble with matrigel 3D culture as it offer too much 'support' for my cells to grow and differentiate. I think it'd be best if we can mimic environment in vivo by mixing purified ECM proteins, in consideration of substrate modification such as glycosylation or of containing some lipid compartment as well.

3dcellculture.com
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For all of you 3D Cell

For all of you 3D Cell Culture enthusiasts....

Check out www.3dcellculture.com! We have a full database of 3D Cell Culture related citations that are searchable using key attributes such as cell type, scaffold material and stimulus.

I hope this helps with your 3D Cell Culture related research.

minylim
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Interesting publication on 3D

Interesting publication on 3D culture...

Paper-supported 3D cell culture for tissue-based bioassays,
States of America." href="javascript:AL_get(this,%20'jour',%20'Proc%20Natl%20Acad%20Sci%20U%20S%20A.');">Proc Natl Acad Sci U S A.
2009 Nov 3;106(44):18457-62. Epub 2009 Oct 21.

3dcellculture.com
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You can find the publication

You can find the publication recommended by minylim at...

www.3dcellculture.com/Search_Results/id/8754

Microtissues
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A great 3D cell culture

A great 3D cell culture solution is the 3D Petri Dish.

Please email me at eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%69%6e%66%6f%40%6d%69%63%72%6f%74%69%73%73%75%65%73%2e%63%6f%6d%22%3e%69%6e%66%6f%40%6d%69%63%72%6f%74%69%73%73%75%65%73%2e%63%6f%6d%3c%2f%61%3e%27%29%3b')) if you need more information about it for your research.