I am currently using ImageJ but am experiencing problems;
- I can only select an area of minimum dimensions around an area of interes, which is larger than I would like
- one of my blots is "dirty", so I find there is a lot of background interference which I cannot resolve
- I am unsure what area to select in the lane selection - are there any rules around whether the background cut off selection must be straight?
- can I analyse both of the doublet bands in the same plot? (as shown in attachment?)
Thanks for any help!