I am planning to do a real-time PCR array based differential gene expression profiling of DNA repair genes. First I am going to knockout gene of our interest (GOI) followed by irradiation (cs 137, gamma radiation) & RNA isolation (24 hrs post irradiation).
I have a problem in choosing the radiation dose.
1).What dose can I use to see a difference in gene expression? Say 5Gy or 10Gy
2).What is the rationale in choosing the dose.
Thanks in advance