Downsream signals of receptor tyrosin kinases in cancer

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nani
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Downsream signals of receptor tyrosin kinases in cancer

I'm trying to illucidate the role of downstream signals of EphB receptor tyrosine kinase in cancer survival.In this regard I would like to know that,within short term exposures of receptor with its cognate ligand ephrin  ( e.g 15min to 2hrs ), would performing MTT assay be sort of appropriate one to determine the effects of a receptor activation and its downstream signals on  overall viability of cancer cells and see if the signals toxic to the cells or not?if so,when is the best time to start MTT assay after the desired time exposure of ligand and receptor has passed?
Please help me find my answer.
Farnaz